Drosophila Melanogaster Laboratory Rearing

Drosophila Melanogaster Laboratory Rearing Abstract   Drosophila melanogaster Linnaeus was reared for single generation in the laboratory on a simple diet of 13gm yeast, 250gm milk, 20gm cellulose tissue and 1L water. Keywords: Drosophila melanogaster, laboratory rearing, developmental stages, artificial diet Introduction Drosophila melanogaster  is commonly known as fruit fly since its always found near unripe and rotted fruit. The fly inspired its genus name from the Latin word means dew loving. This genus could b easily identified by presence of black characteristic hairs over their compound eye. This smallish fly has been paid attention since early 90th in many scientific investigations concerning behavioral and genetic studies. Thomas Hunt Morgan was the first and outstanding biologist studying  Drosophila  early in the 1900s. He was the first to discover sex linked genes and genetic assortment, segregation, and recombination, which put the fly as a model of genetic research. And cause of its smallish size, ease of culture and short generation time, geneticists have been using  the fly ever since. Drosophila sp. is one of the few organisms whose entire genome is known and many of its genes have been identified and used in many other scientific researches. The very fast development enables this organism to develop from eggs to adult stage during 9-12 days in 25 °C. Usually the fly spends five days in the egg  and  larval stages and four days in the pupal  stage. The adult may live for several weeks.  Drosophila sp. should not be reared in high temperatures (e.g. above 30 °C) that will result in sterilization or death of the flies or to low temperatures (e.g. below 10 °C) that also will result in a prolonged life cycle, maybe 57 days, and eventually reduced viability. Their body is usually pale yellow to reddish brown to black in color, with large, red eyes and oval-shaped wings, maybe sometimes it has some distinct black patterns. Also their wing venation is peculiar to the entire family. The body form could be mutated due to culturing conditions, which produces offspring with body characters quietly different from parents.  For instance, higher temperatures might alter the phenotype of the eye and body color, or may change the wing shape and size. The entire genus of Drosophila sp. contains more than 15000 species and is very diverse in appearance, behavior, and breeding habitat (Gerhard Bà ¤chli, 1999-2006). Drosophila  sp. found all over the world, but most of their species are predominant in the tropical regions. They are multivoltine species, and the northern species can hibernate. They breed in various kinds of decaying plants and  fungal  materials. The larvae of some species can also feed on fresh fruit and can sometimes be a pest (  Mark Hoddle).  Some other few species maybe switched in order to be  parasites or  predators. Most of their species are attracted to bait of fermented  fruits or any other fermented odor, but little of them are not attracted to any kind of baits. Males usually congregate at patches of suitable breeding habitats to compete for females. Several  Drosophila  species, such as  D. melanogaster, are closely associated with humans; thereby they are called as  domestic  s pecies. But most of Drosophila sp. have been accidentally to new areas due to anthropogenic activities and fruit transportation (Vilela, 1999; Van der Linde et al., 2006; Castrezana, 2007). The fruit fly varies in their reproductive capacity. D. melanogaster, breeds in rare, limited resources, have  ovaries  that mature 10-20 eggs at a time, so that they can be laid together on one site. Others, those breed in more-abundant but less nutritious substrates, may only lay one egg per day. Only the adult stage feeds on vegetable matters, whereas larvae, the feeding immature stage, feed on yeast and microorganisms present on decaying breeding substrate. Their developmental time vary according to species. It is varied from 7 more than 60 days. Also, it may vary within the same species depending on the environmental factors such as temperature, breeding substrate, and crowding. In this study, D. melanogaster collected and cultured under laboratory conditions, using artificial substrates to investigate different immature stages and the mean developmental time for each stage. Materials and Methods 1. Collecting and Culturing Media Diet mixture of yeast, milk and cellulose was put in plastic bottle. The bottle was cut into two halves, the nick top half, which put inverted on the bottom half. The media recipe was devised as below: Yeast 13gm Milk 250gm Cellulose tissue 20gm H2O 1L This media is blended and placed in plastic collecting bottles containing crumpled paper toweling to prevent flies drowning. The media was stored at 30o for 2-3 days until fermented (Krivshenko 1963), then put outdoor to collect adult. The collecting bottle was checked twice daily (day and night). The collecting bottle left for three days to collect as much as possible of D. melanogaster flies. The collected individuals transferred to culturing bottle under the laboratory conditions (25 °Ã‚ ±5 °C) at plant protection department, Food and Agriculture Sciences College, King Saud University, for conducting the experiment. Flies oviposited in the diet. After 24 hrs, adult flies were removed from the bottles using their positive phototactic response and maintained in 20x20cm cage fitted with a sleeve and fed with 2.5 % sugar solution through cotton wicks. Small 50ml vials with culturing media were used for maintaining small larvae individually and avoid crowding. Number of larval developmental stages and time taken until pupal stage were recorded. Study area and period The study was conducted for 30 days (December, 2010) starting culturing step. Three collecting bottles were used in three selected sites for adult D. melanogaster collection; the educational farm of Food and Agriculture Sciences College, referred as site A, the main building of the College, referred as site B, and the students housing at King Saud University, referred as site C. Results and Discussion Total of 23 adults were collected in the three collecting bottles. Both Sites A and C were negative for adult fly. Eggs laid couldnt be observed since adult females put them in tiny cracks of the culturing media. 301 larvae have been recorded within 24hrs. The mean developmental time for larvae was 7 days to pupation. By the eighth day, all larvae pupate (Fig. 1). Only single adult failed to emerge by the eleventh day. No adult were emerged until the end of month, which means that successful adult emergence took almost 22 days. The rapid development of early immature stages proved favorable conditions for larval development that was not quietly enough for the pupal stage development into adult emergence. The developmental period for  fruit fly varies mainly with temperature, as with many  ectothermic species. The shortest recorded development time (egg to adult), 7 days, is achieved at 28 °C (Ashburner and Thompson 1978, Ashburner et al., 2005). Development time increases at higher temperatures (11 days at 30 °C) cause of heat stress. The ideal development time at 25 °C is supposed to be 8.5 days as Ashburner and Thompson (1978), Ashburner et al., (2005), and Chiang and Hodson (1950) reported in previous investigations. Nevertheless, the findings of the current study showed longer development time especially for late immature stage (pupa). This might be explained, as the temperature of the laboratory was not optimum ( Crowding has no effect on development time since larvae were separated individually in culturing media. More over, under crowding increases the development time (Chiang and Hodson 1950), while the emerging flies are smaller (Chiang and Hodson 1950, Bakker 1961).

Script for Emceeing

Pleasantries†¦. After 41 years of dedicated service to the people of Dagupan City, it is just and right to give honor to someone we call mentor and mother. Mentor of every budding teacher who wanted to follow her footsteps and mother who gives worthwhile lessons in life and motherly advices to teachers like me. Ladies and gentlemen, we are all gathered here for one common purpose†¦to celebrate, give due recognition and reminisce the unforgettable moments we had with our beloved†¦the one and only, Madam Aurora Tiu Domingo or simply Madam Oying! ******************************************************************************* Before everything else, let us all first seek for the Divine providence of our mighty creator with a prayer to be led by Angeline Liwag. ******************************************************************************** Music has always been part of every person’s life. Through its expressive lyrics, they make us smile when we are in our sad mome nts, or even uplift us when we feel so down†¦Folks, Let’s give it up for the Chalk and Eraser Band as they serenade sentimental songs for our beloved Madam Oying. Let’s give them a big hand. ) ******************************************************************************* 41 years is quite long public service. This 41 years is for sure full of accolades from humble beginnings. Though an audio-visual presentation, let’s all see some of the highlights of 41 dedicated years of Madam Oying in Dagupan City Division. ******************************************************************************* Besides being an achiever, Friendly and always at her foot are most of the descriptions given to Madam Oying.It’s time for us to hear testimonies and presentations from the people deem important and influenced by our honoree. (Refer to the program â€Å"Testimonies and Presentations†) ************************************************************************** ******* Let’s now give the floor to Madam Oying for the Response to the testimonies and Presentation ********************************************************************************** Part II-Fellowship

Limitations Of Wired Equivalent Privacy - 2704 Words

Limitations of Wired Equivalent Privacy Presented in IAAS670 Wireless and Mobile Security By Joel Bramblett, Oluwaseun Onibudo, and Michelle Rowton Davenport University October 21, 2014 Nasser Halwani Professor Abstract The Wired Equivalent Privacy algorithm (WEP) is a method of securing IEEE 802.11 wireless internet connections. This algorithm was accepted by the IEEE (The Institute of Electrical and Electronics Engineers) in 1999 and it was subsequently used as the standard for wireless security JATIT (2012). Just over 5 years after its ratification by IEEE, the same body phased it out after some FBI personnel demonstrated how easy it was to crack (in as little as three minutes) (Cheung, 2005). This marked the entry of WPA/WPA2 (Wi-Fi Protected Access), but this is not our area of concentration in this paper. This paper opens with an introduction into the world of WEP and proceeds by concentrating on the various vulnerabilities that led to the abandonment of the WEP as a secure protocol for wireless networks. Our discussion will center on WEP limitations regarding initialization vectors and the method of encryption –RC4 vis-à  -vis the probable fixes for the vulnerabilities. We will conclude by looking at the full WEP operational picture and possible recommendations for securing wireless networks in this modern age. Table of Contents Introduction 4 How WEP Works 4 Limitations 5 Limitations of the Initialization Vectors 5 Source of Initialization vectors 5 IVShow MoreRelated Wireless Networking Essay991 Words   |  4 Pagesshare files and printers, but may not be able access wired LAN resources unless one computer acts as a bridge to connect to a wired LAN. Access points (base stations) can be hardware based or software based. 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Essay on Caring for a Person With Dementia - 2027 Words

Introduction Dementia is an umbrella term used to explain the gradual decline in multiple areas of functions, which includes thinking, perception, communication, memory, languages, reasoning, and the ability to function (Harrison-Dening 2013). Worldwide, 47.5 million people have dementia and there are 7.7 million new cases every year. Alzheimers disease is the most common cause of dementia and may contribute to 60–70% of cases. (Alzheimers society 2014). The complexity of dementia presents a number of behavioural challenges to those who live with dementia and their care providers. Aggressive behaviour seems to be one of the most prevalent challenging behaviours in the different stages of dementia (Weitzel et al 2011). As acute care†¦show more content†¦With major types of dementia, the damaged nerve cells stop fail to keep connections with adjacent cells, and the person’s symptoms may get worse over the period. Common Challenging Behaviours Challenging behaviours are often extremely stressful, which presents managing problems with dementia people as well as their care givers. In general, 80% individuals with dementia most often express the signs of challenging behaviours (McKay2008). The common behavioural disturbances associated with dementia include hallucinations delusions, sleep problems, sundowning, aggression, agitation, wandering, and communication challenges (Zwijsen et al 2014). Aggressive Behaviours Aggression is one of the psychological symptoms of dementia, which depicts a cluster of physical signs that suggest restlessness or emotional distress (McKay2008). This multifaceted major health problem originated from the complex interactions of neurobiological, cultural and environmental factors (Cipriani et al 2011). 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